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代谢细胞生物学研究组

课题组长

姓名：

刘冀珑教授研究员博士生导师, PhD, 教授

职务：

威尼斯432888can副院长

所在院所：

威尼斯432888can

荣誉称号：

教育经历：

1988/09—1992/07，北京林业大学，学士
1992/09—1995/07，中国农业大学，硕士
1997/09—2000/07，中国科学院动物研究所，博士

博士后及工作经历：

2000/07—2002/12，美国康乃狄格大学，博士后
2003/01—2007/08，美国卡内基研究所，博士后
2007/08—2012/01，英国牛津大学，MRC Programme Leader（TENURE-TRACK）
2012/01—2016/05，英国牛津大学，MRC Programme Leader（TENURED）
2016/06—至今，威尼斯432888can，威尼斯432888can，正教授（TENURED）

课题组简介

研究方向：

代谢细胞生物学，细胞蛇

研究内容：

1.以果蝇、酵母菌和人的培养细胞为主要研究材料探索代谢酶（如CTP合成酶和IMP脱氢酶等）在细胞里如何被区域化；

2.研究不同的代谢酶如何组装成细胞蛇，以及细胞蛇和类似的细胞器如何在代谢调控和肿瘤生物学发挥作用；

3.利用不同技术方法和多学科（细胞生物学、生物化学、发育遗传、结构生物学、合成生物学等）理解细胞蛇的结构和功能。

课题组网站：

http://cytoophidia.com

研究成果展示

2010 年包括我们在内的三个研究组首先独立报道了CTP合成酶在细菌，酵母菌和果蝇细胞里形成一种新类型的细胞器“细胞蛇（Cytoophidia）”。细胞蛇在地球上存在至少30亿年，从细菌到人体细胞里均被发现。2020年我们在生命第三域古菌中发现细胞蛇。

果蝇生殖细胞里的细胞蛇（Liu, 2010, 2016）

图1 细胞蛇：细胞里的蛇（Liu, 2010, 2016）

图2 果蝇雌性生殖系统里的细胞蛇（Liu, 2011）

我们实验室以果蝇，酵母以及人的培养细胞为主要研究材料探索代谢酶（如CTP 合成酶和IMP 脱氢酶等）在细胞里如何被区域化。我们使用结构生物学手段如冷冻电镜技术解析细胞蛇近原子分辨率的结构。

图3 GTP结合CTP合成酶（Zhou, Guo, Chang et al., 2021）

图片设计：Julie Liu

此外，我们也积极开发CRISPR衍生技术。最近，我们与同事们合作开发了捕捉RNA-蛋白质相互作用的CRUIS。

图4 世界第一只用CRISPR技术生产的y w双基因突变果蝇（Bassett and Liu, 2014）

代表性论文（*第一作者，#通讯作者）

1. Liu, JL*; Murphy, C; Buszczak, M; Clatterbuck, S; Goodman, R; Gall, JG^#.The Drosophila melanogaster Cajal body.JOURNAL OF CELL BIOLOGY. 2006. 172(6):875-884.
2. Liu, Ji-Long^#*.Intracellular compartmentation of CTP synthase in Drosophila.JOURNAL OF GENETICS AND GENOMICS. 2010. 37(5):281-296.
3. Bassett, Andrew R.^#*; Tibbit, Charlotte; Ponting, Chris P.; Liu, Ji-Long^#.Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System.CELL REPORTS. 2013. 4(1):220-228.
4. Ghosh, Sanjay^#*; Tibbit, Charlotte; Liu, Ji-Long^#.Effective knockdown of Drosophila long non-coding RNAs by CRISPR interference.NUCLEIC ACIDS RESEARCH. 2016. 44(9).
5. Aughey, Gabriel N.*; Grice, Stuart J.; Liu, Ji-Long^#.The Interplay between Myc and CTP Synthase in Drosophila.PLOS GENETICS. 2016. 12(2).
6. Liu, Ji-Long^#*.The Cytoophidium and Its Kind: Filamentation and Compartmentation of Metabolic Enzymes.ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY. 2016. 32:349-372.
7. Huang, Yong*; Wang, Jin-Jun; Ghosh, Sanjay^#; Liu, Ji-Long^#.Critical roles of CTP synthase N-terminal in cytoophidium assembly.EXPERIMENTAL CELL RESEARCH. 15 May 2017. 354(2):122-133.
8. Chang, Chia-Chun*; Jeng, Yung-Ming; Peng, Min; Keppeke, Gerson Dierley; Sung, Li-Ying^#; Liu, Ji-Long^#.CTP synthase forms the cytoophidium in human hepatocellular carcinoma.EXPERIMENTAL CELL RESEARCH. 15 Dec 2017. 361(2):292-299.
9. Keppeke, Gerson Dierley*; Chang, Chia Chun*; Peng, Min; Chen, Li-Yu; Lin, Wei-Cheng; Pai, Li-Mei; Andrade, Luis Eduardo Coelho; Sung, Li-Ying^#; Liu, Ji-Long^#.IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.CELL DIVISION. 15 Jun 2018. 13.
10. Chang, Chia-Chun*; Keppeke, Gerson D.*; Sung, Li-Ying^#; Liu, Ji-Long^#.Interfilament interaction between IMPDH and CTPS cytoophidia.FEBS JOURNAL. Oct 2018. 285(20):3753-3768.
11. Zhang, Shanshan*; Ding, Kang; Shen, Qing-Ji; Zhao, Suwen; Liu, Ji-Long^#.Filamentation of asparagine synthetase in Saccharomyces cerevisiae.PLOS GENETICS. Oct 2018. 14(10).
12. Li, Hui*; Ye, Fangfu; Ren, Jing-Yi; Wang, Peng-Ye; Du, Li-Lin; Liu, Ji-Long^#.Active transport of cytoophidia in Schizosaccharomyces pombe.FASEB JOURNAL. Nov 2018. 32(11):5891-5898.
13. Sun, Zhe*; Liu, Ji-Long^#.mTOR-S6K1 pathway mediates cytoophidium assembly.JOURNAL OF GENETICS AND GENOMICS. Feb 2019. 46(2):65-74.
14. Wu, Zheng*; Liu, Ji-Long^#.Cytoophidia respond to nutrient stress in Drosophila.EXPERIMENTAL CELL RESEARCH. 15 Mar 2019. 376(2):159-167.
15. Sun, Zhe*; Liu, Ji-Long^#.Forming cytoophidia prolongs the half-life of CTP synthase.CELL DISCOVERY. 18 Jun 2019. 5.
16. Andreadis, Christos*; Hulme, Lydia; Wensley, Katherine; Liu, Ji-Long^#.The TOR pathway modulates cytoophidium formation in Schizosaccharomyces pombe.JOURNAL OF BIOLOGICAL CHEMISTRY. 2019. 294(40):14686-14703.
17. Zhang, Jing*; Liu, Ji-Long^#.Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe.JOURNAL OF GENETICS AND GENOMICS. 20 Sep 2019. 46(9):423-432.
18. Zhou, Xian*; Guo, Chen-Jun*; Hu, Huan-Huan; Zhong, Jiale; Sun, Qianqian; Liu, Dandan; Zhou, Shuang; Chang, Chia Chun; Liu, Ji-Long^#.Drosophila CTP synthase can form distinct substrate- and product-bound filaments.JOURNAL OF GENETICS AND GENOMICS. 20 Nov 2019. 46(11):537-545.
19. Zhang Ziheng*; Sun Weiping*; Shi Tiezhu*; Lu Pengfei^#; Zhuang Min^#; Liu Ji-Long^#.Capturing RNA-protein Interaction via CRUIS.NUCLEIC ACIDS RESEARCH. 06 Mar 2020. 48(9):e52.
20. Zhang, Bo*; Tastan, Omur Y.*; Zhou, Xian*; Guo, Chen-Jun; Liu, Xuyang; Thind, Aaron; Hu, Huan-Huan; Zhao, Suwen; Liu, Ji-Long^#.The proline synthesis enzyme P5CS forms cytoophidia in Drosophila.JOURNAL OF GENETICS AND GENOMICS. 20 Mar 2020. 47(3):131-143.
21. Zhou, Shuang*; Xiang, Hua; Liu, Ji-Long^#.CTP synthase forms cytoophidia in archaea.JOURNAL OF GENETICS AND GENOMICS. 20 Apr 2020. 47(4):213-223.
22. Liu, Ji-Long^#*; Gall, Joseph G.^#.Cold shock induces novel nuclear bodies in Xenopus oocytes.EXPERIMENTAL CELL RESEARCH. Jan 2021. 398(1).
23. Li, Yi-Lan*; Liu, Ji-Long^#.Hypoosmolality impedes cytoophidium integrity during nitrogen starvation.YEAST. Jan 2021. 38(4):276-289.
24. Wang, Qiao-Qi*; Zhao, Peiyao A.; Tastan, Omur Y.; Liu, Ji-Long.Polarised maintenance of cytoophidia in Drosophila follicle epithelia.EXPERIMENTAL CELL RESEARCH. 2021. 402(2).
25. Zhou, Xian*; Guo, Chen-Jun*; Chang, Chia-Chun*; Zhong, Jiale; Hu, Huan-Huan; Lu, Guang-Ming; Liu, Ji-Long^#.Structural basis for ligand binding modes of CTP synthase.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2021. 118(30).
26. Zhou, Youfang*; Liu, Jingnan; Zhang, Yuanbing; Liu, Ji-Long.Drosophila intestinal homeostasis requires CTP synthase.EXPERIMENTAL CELL RESEARCH. 2021. 408(1).